Toxoplasma gondii and Neospora caninum in wild small mammals: Seroprevalence, DNA detection and genotyping
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
27198782
DOI
10.1016/j.vetpar.2016.04.018
PII: S0304-4017(16)30126-1
Knihovny.cz E-resources
- Keywords
- Czech Republic, Genotype, Neosporosis, Rodents, Toxoplasmosis,
- MeSH
- Animals, Wild MeSH
- Genotype MeSH
- Rodentia * MeSH
- Coccidiosis epidemiology parasitology veterinary MeSH
- Microsatellite Repeats MeSH
- Neospora genetics isolation & purification MeSH
- DNA, Protozoan isolation & purification MeSH
- Shrews * MeSH
- Seroepidemiologic Studies MeSH
- Toxoplasma genetics isolation & purification MeSH
- Toxoplasmosis, Animal epidemiology parasitology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- DNA, Protozoan MeSH
Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002-2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5%) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay in one A. flavicolis (0.4%). Three of 427 (0.7%) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n=102) were negative for both T. gondii and N. caninum. The three liver samples positive for T. gondii DNA (two from A. flavicollis and one from A. sylvaticus) were genotyped by 15 microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic.
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