Construction and characterization of a BAC library for functional genomics in Xenopus tropicalis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27256582
DOI
10.1016/j.ydbio.2016.05.015
PII: S0012-1606(16)30050-1
Knihovny.cz E-zdroje
- Klíčová slova
- Amphibian, BAC library, Genomics, Xenopus tropicalis,
- MeSH
- genomika metody MeSH
- genová knihovna * MeSH
- hybridizace in situ fluorescenční MeSH
- játra chemie MeSH
- mapování chromozomů MeSH
- proteiny Xenopus genetika MeSH
- sekvenční analýza DNA MeSH
- umělé bakteriální chromozomy genetika MeSH
- Xenopus genetika MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- proteiny Xenopus MeSH
Large insert genomic DNA libraries are useful resources for genomic studies. Although the genome of Xenopus tropicalis stands as the amphibian reference genome because it benefitted from large-scale sequencing studies, physical mapping resources such as BAC libraries are lagging behind. Here we present the construction and characterization of a BAC library that covers the whole X. tropicalis genome. We prepared this BAC library from the genomic DNA of X. tropicalis females of the Adiopodoume strain. We characterized BAC clones by screening for specific loci, by chromosomal localization using FISH and by systematic BAC end sequencing. The median insert size is about 110kbp and the library coverage is around six genome equivalents. We obtained a total of 163,787 BAC end sequences with mate pairs for 77,711 BAC clones. We mapped all BAC end sequences to the reference X. tropicalis genome assembly to enable the identification of BAC clones covering specific loci. Overall, this BAC library resource complements the knowledge of the X. tropicalis genome and should further promote its use as a reference genome for developmental biology studies and amphibian comparative genomics.
CEA Institut de Génomique Genoscope Evry France
Department of Cell Biology Faculty of Science Charles University Prague Prague Czech Republic
Citace poskytuje Crossref.org
