The integration of cyanide hydratase and tyrosinase catalysts enables effective degradation of cyanide and phenol in coking wastewaters
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27328365
DOI
10.1016/j.watres.2016.06.016
PII: S0043-1354(16)30454-7
Knihovny.cz E-zdroje
- Klíčová slova
- Coking wastewater, Cresols, Cyanide, Cyanide hydratase, Phenol, Tyrosinase,
- MeSH
- fenol metabolismus MeSH
- fenoly metabolismus MeSH
- koks MeSH
- kyanidy metabolismus MeSH
- odpadní voda * MeSH
- tyrosinasa MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fenol MeSH
- fenoly MeSH
- koks MeSH
- kyanidy MeSH
- odpadní voda * MeSH
- tyrosinasa MeSH
The aim of this study was to design an effective method for the bioremediation of coking wastewaters, specifically for the concurrent elimination of their highly toxic components - cyanide and phenols. Almost full degradation of free cyanide (0.32-20 mM; 8.3-520 mg L(-1)) in the model and the real coking wastewaters was achieved by using a recombinant cyanide hydratase in the first step. The removal of cyanide, a strong inhibitor of tyrosinase, enabled an effective degradation of phenols by this enzyme in the second step. Phenol (16.5 mM, 1,552 mg L(-1)) was completely removed from a real coking wastewater within 20 h and cresols (5.0 mM, 540 mg L(-1)) were removed by 66% under the same conditions. The integration of cyanide hydratase and tyrosinase open up new possibilities for the bioremediation of wastewaters with complex pollution.
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