RGDS- and SIKVAVS-Modified Superporous Poly(2-hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications
Language English Country Germany Media print-electronic
Document type Journal Article
- Keywords
- IKVAV, RGD, peptide, poly(2-hydroxyethyl methacrylate), stem cell,
- MeSH
- Cell Line MeSH
- Humans MeSH
- Methylmethacrylates chemistry pharmacology MeSH
- Mesenchymal Stem Cells cytology MeSH
- Neural Stem Cells cytology metabolism MeSH
- Oligopeptides * chemistry pharmacology MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- arginyl-glycyl-aspartyl-serine MeSH Browser
- hydroxymethylmethacrylate MeSH Browser
- Methylmethacrylates MeSH
- Oligopeptides * MeSH
- seryl-isoleucyl-lysyl-valyl-alanyl-valinamide MeSH Browser
Three-dimensional hydrogel supports for mesenchymal and neural stem cells (NSCs) are promising materials for tissue engineering applications such as spinal cord repair. This study involves the preparation and characterization of superporous scaffolds based on a copolymer of 2-hydroxyethyl and 2-aminoethyl methacrylate (HEMA and AEMA) crosslinked with ethylene dimethacrylate. Ammonium oxalate is chosen as a suitable porogen because it consists of needle-like crystals, allowing their parallel arrangement in the polymerization mold. The amino group of AEMA is used to immobilize RGDS and SIKVAVS peptide sequences with an N-γ-maleimidobutyryloxy succinimide ester linker. The amount of the peptide on the scaffold is determined using 125 I radiolabeled SIKVAVS. Both RGDS- and SIKVAVS-modified poly(2-hydroxyethyl methacrylate) scaffolds serve as supports for culturing human mesenchymal stem cells (MSCs) and human fetal NSCs. The RGDS sequence is found to be better for MSC and NSC proliferation and growth than SIKVAVS.
References provided by Crossref.org
Biomaterials and Magnetic Stem Cell Delivery in the Treatment of Spinal Cord Injury
