Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
27497872
DOI
10.1016/j.dci.2016.07.020
PII: S0145-305X(16)30237-3
Knihovny.cz E-resources
- Keywords
- Blind ileal loops, Germfree animals, Ileal Peyers patches, Immunoglobulin A,
- MeSH
- Lymphocyte Activation MeSH
- B-Lymphocytes physiology MeSH
- Cell Differentiation MeSH
- Germ-Free Life MeSH
- Ileum immunology MeSH
- Immunoglobulin A, Secretory genetics MeSH
- Immunoglobulin M genetics MeSH
- Immunologic Memory MeSH
- Cells, Cultured MeSH
- Animals, Newborn MeSH
- Peyer's Patches immunology MeSH
- Swine immunology MeSH
- Immunoglobulin Class Switching MeSH
- Probiotics administration & dosage MeSH
- Cell Proliferation MeSH
- Antibody Diversity MeSH
- Gastrointestinal Microbiome immunology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Immunoglobulin A, Secretory MeSH
- Immunoglobulin M MeSH
The ileal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised ∼70% while in controls, IgA levels were elevated 5-fold and comprised ∼70% of total Igs. Loop piglets also had reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum.
College of Animal Science and Technology Northwest A and F University Yangling China
Department of Microbiology Carver College of Medicine University of Iowa Iowa City IA USA
Department of Veterinary Sciences South Dakota State University Brooking SD USA
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