Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
27497872
DOI
10.1016/j.dci.2016.07.020
PII: S0145-305X(16)30237-3
Knihovny.cz E-zdroje
- Klíčová slova
- Blind ileal loops, Germfree animals, Ileal Peyers patches, Immunoglobulin A,
- MeSH
- aktivace lymfocytů MeSH
- B-lymfocyty fyziologie MeSH
- buněčná diferenciace MeSH
- gnotobiologické modely MeSH
- ileum imunologie MeSH
- imunoglobulin A sekreční genetika MeSH
- imunoglobulin M genetika MeSH
- imunologická paměť MeSH
- kultivované buňky MeSH
- novorozená zvířata MeSH
- Peyerovy pláty imunologie MeSH
- prasata imunologie MeSH
- přesmyk imunoglobulinových tříd MeSH
- probiotika aplikace a dávkování MeSH
- proliferace buněk MeSH
- rozmanitost protilátek MeSH
- střevní mikroflóra imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- imunoglobulin A sekreční MeSH
- imunoglobulin M MeSH
The ileal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised ∼70% while in controls, IgA levels were elevated 5-fold and comprised ∼70% of total Igs. Loop piglets also had reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum.
College of Animal Science and Technology Northwest A and F University Yangling China
Department of Microbiology Carver College of Medicine University of Iowa Iowa City IA USA
Department of Veterinary Sciences South Dakota State University Brooking SD USA
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