Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27529132
DOI
10.2217/epi-2016-0044
Knihovny.cz E-resources
- Keywords
- DNA methylation, Illumina Human Methylation 450 BeadChip, rectal cancer,
- MeSH
- Alcohol Oxidoreductases genetics MeSH
- Epigenesis, Genetic * MeSH
- Homeodomain Proteins genetics MeSH
- Humans MeSH
- DNA Methylation * MeSH
- Mitochondrial Proteins genetics MeSH
- Biomarkers, Tumor genetics MeSH
- Rectal Neoplasms genetics MeSH
- Proto-Oncogene Protein c-fli-1 genetics MeSH
- Proto-Oncogene Proteins genetics MeSH
- Intestinal Mucosa metabolism MeSH
- Carrier Proteins genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alcohol Oxidoreductases MeSH
- BPIFB6 protein, human MeSH Browser
- FLI1 protein, human MeSH Browser
- Homeodomain Proteins MeSH
- hydroxyacid-oxoacid transhydrogenase MeSH Browser
- Mitochondrial Proteins MeSH
- Biomarkers, Tumor MeSH
- Proto-Oncogene Protein c-fli-1 MeSH
- Proto-Oncogene Proteins MeSH
- TLX1 protein, human MeSH Browser
- Carrier Proteins MeSH
AIM: The aim of the present study is to address a genome-wide search for novel methylation biomarkers in the rectal cancer (RC), as only scarce information on methylation profile is available. MATERIALS & METHODS: We analyzed methylation status in 25 pairs of RC and adjacent healthy mucosa using the Illumina Human Methylation 450 BeadChip. RESULTS: We found significantly aberrant methylation in 33 genes. After validation of our results by pyrosequencing, we found a good agreement with our findings. The BPIL3 and HBBP1 genes resulted hypomethylated in RC, whereas TIFPI2, ADHFE1, FLI1 and TLX1 were hypermethylated. An external validation by TCGA datasets confirmed the results. CONCLUSION: Our study, with external validation, has demonstrated the feasibility of using specific methylated DNA signatures for developing biomarkers in RC.
Biomedical Centre Faculty of Medicine in Pilsen Charles University Czech Republic
Department of Laboratory Medicine Örebro University; Örebro Sweden
Department of Surgery General University Hospital Prague Czech Republic
Human Genetics Foundation Torino Italy
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
References provided by Crossref.org
