Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27529132
DOI
10.2217/epi-2016-0044
Knihovny.cz E-zdroje
- Klíčová slova
- DNA methylation, Illumina Human Methylation 450 BeadChip, rectal cancer,
- MeSH
- alkoholoxidoreduktasy genetika MeSH
- epigeneze genetická * MeSH
- homeodoménové proteiny genetika MeSH
- lidé MeSH
- metylace DNA * MeSH
- mitochondriální proteiny genetika MeSH
- nádorové biomarkery genetika MeSH
- nádory rekta genetika MeSH
- protoonkogenní protein c-fli-1 genetika MeSH
- protoonkogenní proteiny genetika MeSH
- střevní sliznice metabolismus MeSH
- transportní proteiny genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkoholoxidoreduktasy MeSH
- BPIFB6 protein, human MeSH Prohlížeč
- FLI1 protein, human MeSH Prohlížeč
- homeodoménové proteiny MeSH
- hydroxyacid-oxoacid transhydrogenase MeSH Prohlížeč
- mitochondriální proteiny MeSH
- nádorové biomarkery MeSH
- protoonkogenní protein c-fli-1 MeSH
- protoonkogenní proteiny MeSH
- TLX1 protein, human MeSH Prohlížeč
- transportní proteiny MeSH
AIM: The aim of the present study is to address a genome-wide search for novel methylation biomarkers in the rectal cancer (RC), as only scarce information on methylation profile is available. MATERIALS & METHODS: We analyzed methylation status in 25 pairs of RC and adjacent healthy mucosa using the Illumina Human Methylation 450 BeadChip. RESULTS: We found significantly aberrant methylation in 33 genes. After validation of our results by pyrosequencing, we found a good agreement with our findings. The BPIL3 and HBBP1 genes resulted hypomethylated in RC, whereas TIFPI2, ADHFE1, FLI1 and TLX1 were hypermethylated. An external validation by TCGA datasets confirmed the results. CONCLUSION: Our study, with external validation, has demonstrated the feasibility of using specific methylated DNA signatures for developing biomarkers in RC.
Biomedical Centre Faculty of Medicine in Pilsen Charles University Czech Republic
Department of Laboratory Medicine Örebro University; Örebro Sweden
Department of Surgery General University Hospital Prague Czech Republic
Human Genetics Foundation Torino Italy
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
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