Seminal plasma (SP) and ovarian fluid (OF) plays an important role as storage media to prevent the activation of gametes both in vivo and under artificial conditions. The objectives of this study were to quantify gamete biochemistry and explore correlations among quantitative characteristics of SP, OF and sperm performance traits of Ide Leuciscus idus and Northern pike Esox lucius. Generally, Na+ , K+ and Cl- were found to be the most dominating ions, although concentrations of K+ were higher in SP, while Na+ and Cl- concentrations were higher in OF for both species. Several significant correlations among the biochemical properties such as total protein, glucose, osmolality, cholesterol, K+ , Ca2+ , Cl- and Mg2+ were observed for SP and OF. Total protein content of Ide SP was positively correlated with sperm activity traits (r ≥ .89, p ≤ .05), while K+ concentration was negatively correlated with sperm traits (r ≥ -.89, p ≤ .05). Moreover, Ca2+ concentration in Northern pike SP was positively correlated with the percentage of sperm motility (r = . 98, p < .01). In conclusion, these results can be used to better understand the biochemistry of SP and OF, improve methods for short- and long-term storage of gametes and standardize fertilization protocols.

Department of Lake and River Fisheries Warmia and Mazury University Olsztyn Poland

DTU Aqua National Institute of Aquatic Resources Section for Marine Ecology and Oceanography Technical University of Denmark Charlottenlund Denmark

Faculty of Fisheries and Protection of Waters University of South Bohemia in Ceske Budejovice South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Research Institute of Fish Culture and Hydrobiology Vodňany Czech Republic

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