MYD88 and functionally related genes are associated with multiple infections in a model population of Kenyan village dogs
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
27655108
DOI
10.1007/s11033-016-4078-8
PII: 10.1007/s11033-016-4078-8
Knihovny.cz E-resources
- Keywords
- Associations, Immunity-related genes, Infectious diseases, Kenyan village dogs,
- MeSH
- Genetic Predisposition to Disease MeSH
- Genetic Association Studies MeSH
- Polymorphism, Single Nucleotide MeSH
- Myeloid Differentiation Factor 88 genetics MeSH
- Protozoan Infections, Animal genetics MeSH
- Dogs MeSH
- Distemper genetics MeSH
- Sequence Analysis, DNA MeSH
- Toll-Like Receptor 4 genetics MeSH
- Rural Population MeSH
- Animals MeSH
- Check Tag
- Dogs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Kenya MeSH
- Names of Substances
- Myeloid Differentiation Factor 88 MeSH
- Toll-Like Receptor 4 MeSH
The purpose of this study was to seek associations between immunity-related molecular markers and endemic infections in a model population of African village dogs from Northern Kenya with no veterinary care and no selective breeding. A population of village dogs from Northern Kenya composed of three sub-populations from three different areas (84, 50 and 55 dogs) was studied. Canine distemper virus (CDV), Hepatozoon canis, Microfilariae (Acantocheilonema dracunculoides, Acantocheilonema reconditum) and Neospora caninum were the pathogens studied. The presence of antibodies (CDV, Neospora), light microscopy (Hepatozoon) and diagnostic PCR (Microfilariae) were the methods used for diagnosing infection. Genes involved in innate immune mechanisms, NOS3, IL6, TLR1, TLR2, TLR4, TLR7, TLR9, LY96, MYD88, and three major histocompatibility genes class II genes were selected as candidates. Single nucleotide polymorphism (SNP) markers were detected by Sanger sequencing, next generation sequencing and PCR-RFLP. The Fisher´s exact test for additive and non-additive models was used for association analyses. Three SNPs within the MYD88 gene and one TLR4 SNP marker were associated with more than one infection. Combined genotypes and further markers identified by next generation sequencing confirmed associations observed for individual genes. The genes associated with infection and their combinations in specific genotypes match well our knowledge on their biological role and on the role of the relevant biological pathways, respectively. Associations with multiple infections observed between the MYD88 and TLR4 genes suggest their involvement in the mechanisms of anti-infectious defenses in dogs.
State Veterinary Institute Prague Sidlistni 136 24 165 03 Praha Czech Republic
Vétérinaires Sans Frontières Czech Republic Palackeho 1 61242 Brno Czech Republic
See more in PubMed
Tissue Antigens. 2007 Apr;69 Suppl 1:292-6 PubMed
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13903-8 PubMed
Infect Immun. 2002 Jun;70(6):2763-71 PubMed
Biol Lett. 2008 Apr 23;4(2):228-31 PubMed
Nat Rev Genet. 2005 Feb;6(2):95-108 PubMed
Mol Immunol. 2015 Jul;66(1):89-96 PubMed
Trends Ecol Evol. 2006 Aug;21(8):433-8 PubMed
Proc R Soc Lond B Biol Sci. 1983 Oct 22;219(1216):281-313 PubMed
Viruses. 2014 May 26;6(5):2214-41 PubMed
Genet Sel Evol. 2002 May-Jun;34(3):275-305 PubMed
Int J Evol Biol. 2013;2013:204240 PubMed
Mol Biochem Parasitol. 2010 Dec;174(2):117-27 PubMed
Brief Bioinform. 2008 Jan;9(1):1-13 PubMed
Geospat Health. 2013 May;7(2):409-11 PubMed
Clin Microbiol Rev. 2011 Jul;24(3):490-7 PubMed
Bioinformatics. 2012 Oct 1;28(19):2537-9 PubMed
Trends Genet. 2012 May;28(5):233-43 PubMed
Proc Biol Sci. 2010 Apr 7;277(1684):979-88 PubMed
Vet J. 2011 Aug;189(2):220-6 PubMed
J Neuroimmunol. 1999 Jun 1;97(1-2):102-9 PubMed
Vet Immunol Immunopathol. 1999 Aug 2;69(2-4):101-11 PubMed
Immunogenetics. 2003 Apr;55(1):23-8 PubMed
J Immunol. 2006 Sep 15;177(6):3515-9 PubMed
J Immunol. 2002 Oct 1;169(7):3863-8 PubMed
Anim Genet. 2011 Apr;42(2):181-90 PubMed
Trends Ecol Evol. 1988 Oct;3(10):254-9 PubMed
J Infect Dis. 2013 Jan 1;207(1):152-63 PubMed
J Immunol. 2005 Nov 15;175(10):6723-32 PubMed
Dis Markers. 2009;27(3):173-86 PubMed
Cell Microbiol. 2005 Nov;7(11):1603-15 PubMed
PLoS One. 2012;7(2):e31820 PubMed
Mol Ecol. 2012 Apr;21(7):1632-46 PubMed
PLoS One. 2014 Apr 25;9(4):e96121 PubMed
Vet Microbiol. 2000 Mar 15;72(3-4):217-27 PubMed
Vet Parasitol. 2011 Dec 15;182(2-4):230-8 PubMed
Microbes Infect. 2011 Mar;13(3):226-31 PubMed
PLoS One. 2015 Sep 08;10(9):e0136749 PubMed
J Immunol. 2013 Nov 1;191(9):4740-7 PubMed
Am J Pathol. 2005 May;166(5):1419-26 PubMed
Science. 2010 Oct 8;330(6001):243-6 PubMed
Tissue Antigens. 2007 Apr;69 Suppl 1:272-88 PubMed
Tissue Antigens. 2006 Dec;68(6):502-8 PubMed
Mamm Genome. 2011 Feb;22(1-2):100-10 PubMed
Curr Biol. 2005 Jun 7;15(11):1022-7 PubMed
PLoS Genet. 2011 Oct;7(10):e1002343 PubMed
J Immunol. 2005 Jan 1;174(1):284-93 PubMed
Curr Opin Immunol. 2014 Oct;30:32-8 PubMed
Nat Genet. 2005 Nov;37(11):1243-6 PubMed
Curr Opin Immunol. 2014 Oct;30:9-16 PubMed
Tissue Antigens. 2014 Sep;84(3):271-6 PubMed
Nat Rev Genet. 2006 Oct;7(10):781-91 PubMed
Genome Biol. 2011;12(2):216 PubMed
Hum Immunol. 2015 Oct;76(10):717-23 PubMed
Tissue Antigens. 2011 Jan;77(1):30-5 PubMed
Infect Immun. 2004 Apr;72(4):1920-8 PubMed
Immunol Res. 2015 Jun;62(2):234-52 PubMed
