Transmembrane adaptor proteins (TRAPs) are important organisers for the transduction of immunoreceptor-mediated signals. Prr7 is a TRAP that regulates T cell receptor (TCR) signalling and potently induces cell death when overexpressed in human Jurkat T cells. Whether endogenous Prr7 has a similar functional role is currently unknown. To address this issue, we analysed the development and function of the immune system in Prr7 knockout mice. We found that loss of Prr7 partially impairs development of single positive CD4+ T cells in the thymus but has no effect on the development of other T cell subpopulations, B cells, NK cells, or NKT cells. Moreover, Prr7 does not affect the TCR signalling pathway as T cells derived from Prr7 knockout and wild-type animals and stimulated in vitro express the same levels of the activation marker CD69, and retain their ability to proliferate and activate induced cell death programs. Importantly, Prr7 knockout mice retained the capacity to mount a protective immune response when challenged with Listeria monocytogenes infection in vivo. In addition, T cell effector functions (activation, migration, and reactivation) were normal following induction of experimental autoimmune encephalomyelitis (EAE) in Prr7 knockout mice. Collectively, our work shows that loss of Prr7 does not result in a major immune system phenotype and suggests that Prr7 has a dispensable function for TCR signalling.

Department of Immune Control Helmholtz Center of Infection Research Braunschweig Germany

Dominick P Purpura Department of Neuroscience Albert Einstein College of Medicine Bronx New York United States of America

Group of Adaptive Immunity Institute of Molecular Genetics CAS Prague Czech Republic

Institute of Medical Microbiology Otto von Guericke University Germany

Institute of Molecular and Clinical Immunology Otto von Guericke University Magdeburg Germany

Leibniz Group 'Dendritic Organelles and Synaptic Function' University Medical Center Hamburg Eppendorf Center for Molecular Neurobiology Hamburg Germany

Organ Specific Immune Regulation Helmholtz Center of Infection Research Braunschweig Germany

RG Neuroplasticity Leibniz Institute for Neurobiology Magdeburg Germany

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