Effect of O-methyl-β-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of l-glutamate in brain nerve terminals
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27721167
DOI
10.1016/j.colsurfb.2016.10.007
PII: S0927-7765(16)30710-X
Knihovny.cz E-zdroje
- Klíčová slova
- Cholesterol, Glutamate, Maghemite nanoparticles, Nerve terminals, O-Methyl-β-cyclodextrin,
- MeSH
- beta-cyklodextriny chemie farmakologie MeSH
- biologický transport účinky léků MeSH
- buněčná membrána účinky léků metabolismus MeSH
- cholesterol izolace a purifikace metabolismus farmakologie MeSH
- kinetika MeSH
- krysa rodu Rattus MeSH
- kyselina glutamová metabolismus MeSH
- magnetické nanočástice chemie MeSH
- membránové potenciály účinky léků MeSH
- mozek účinky léků metabolismus MeSH
- potkani Wistar MeSH
- presynaptická zakončení účinky léků metabolismus MeSH
- radioizotopy uhlíku MeSH
- silany chemie MeSH
- synaptozomy účinky léků metabolismus MeSH
- železité sloučeniny chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beta-cyklodextriny MeSH
- cholesterol MeSH
- ferric oxide MeSH Prohlížeč
- kyselina glutamová MeSH
- magnetické nanočástice MeSH
- methyl-beta-cyclodextrin MeSH Prohlížeč
- radioizotopy uhlíku MeSH
- silany MeSH
- železité sloučeniny MeSH
Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals nonspecifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol-depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-β-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (γ-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic γ-Fe2O3 provides a relatively stable colloid product containing 48μmol of MCDg-1. MCD-modified γ-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of l-[14C]glutamate and increase the extracellular l-[14C]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution; moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol.
Citace poskytuje Crossref.org
