A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland
Language English Country United States Media print
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
R03 HD060807
NICHD NIH HHS - United States
Wellcome Trust - United Kingdom
- Keywords
- 3D culture, Branching morphogenesis, Extracellular matrix, Fibroblasts, Mammary epithelium, Matrigel, Organoids, Organotypic assay, Paracrine signaling,
- MeSH
- Cellular Microenvironment physiology MeSH
- Stromal Cells cytology MeSH
- Epithelium physiology MeSH
- Epithelial Cells cytology MeSH
- Extracellular Matrix physiology MeSH
- Fibroblasts cytology MeSH
- Coculture Techniques methods MeSH
- Cells, Cultured MeSH
- Mammary Glands, Animal cytology MeSH
- Morphogenesis physiology MeSH
- Mice MeSH
- Signal Transduction physiology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
References provided by Crossref.org
Fibroblast-induced mammary epithelial branching depends on fibroblast contractility
An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast
Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks
