A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
R03 HD060807
NICHD NIH HHS - United States
Wellcome Trust - United Kingdom
- Klíčová slova
- 3D culture, Branching morphogenesis, Extracellular matrix, Fibroblasts, Mammary epithelium, Matrigel, Organoids, Organotypic assay, Paracrine signaling,
- MeSH
- buněčné mikroprostředí fyziologie MeSH
- buňky stromatu cytologie MeSH
- epitel fyziologie MeSH
- epitelové buňky cytologie MeSH
- extracelulární matrix fyziologie MeSH
- fibroblasty cytologie MeSH
- kokultivační techniky metody MeSH
- kultivované buňky MeSH
- mléčné žlázy zvířat cytologie MeSH
- morfogeneze fyziologie MeSH
- myši MeSH
- signální transdukce fyziologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
Citace poskytuje Crossref.org
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