Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27803935
PubMed Central
PMC5075626
DOI
10.1155/2016/4214328
Knihovny.cz E-resources
- MeSH
- Tissue Donors statistics & numerical data MeSH
- Length of Stay statistics & numerical data MeSH
- Diabetes Mellitus, Type 1 surgery MeSH
- Edema epidemiology MeSH
- Hospitalization MeSH
- Immunohistochemistry MeSH
- Body Mass Index MeSH
- Insulin metabolism MeSH
- Islets of Langerhans metabolism surgery MeSH
- Humans MeSH
- Pancreas MeSH
- Retrospective Studies MeSH
- Cold Ischemia statistics & numerical data MeSH
- Islets of Langerhans Transplantation methods MeSH
- Vasoconstrictor Agents therapeutic use MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Insulin MeSH
- Vasoconstrictor Agents MeSH
Variability of pancreatic donors may significantly impact the success of islet isolation. The aim of this study was to evaluate donor factors associated with isolation failure and to investigate whether immunohistology could contribute to organ selection. Donor characteristics were evaluated for both successful (n = 61) and failed (n = 98) islet isolations. Samples of donor pancreatic tissue (n = 78) were taken for immunohistochemical examination. Islet isolations with 250000 islet equivalents were considered successful. We confirmed that BMI of less than 25 kg/m2 (P < 0.001), cold ischemia time more than 8 hours (P < 0.01), hospitalization longer than 96 hours (P < 0.05), higher catecholamine doses (P < 0.05), and edematous pancreases (P < 0.01) all unfavorably affected isolation outcome. Subsequent immunohistochemical examination of donor pancreases confirmed significant differences in insulin-positive areas (P < 0.001). ROC analyses then established that the insulin-positive area in the pancreas could be used to predict the likely success of islet isolation (P < 0.001). At the optimal cutoff point (>1.02%), sensitivity and specificity were 89% and 76%, respectively. To conclude, while the insulin-positive area, determined preislet isolation, as a single variable, is sufficient to predict isolation outcome and helps to improve the success of this procedure, its combination with the established donor scoring system might further improve organ selection.
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