Colorectal cancer is the third most common cancer. Approximately 20% of patients have at the time of presentation metastasized colorectal cancer, which is incurable in ~80% of cases. The present case report describes a typical case diagnosed with an advanced invasive colorectal adenocarcinoma, with two suspect hypodense lesions in the liver, as revealed by sonography. Judged inoperable for a curative outcome by radical resection, the patient was treated with a novel surgical technique based on stimulating the immune system, termed 'autologous tumor immunizing devascularization' (ATID). The tumor was isolated from its surroundings by ligature of arteries and veins, and subsequently the completely devascularized tumor was left in situ. The distal part of the rectum was closed, and a stoma was made from the proximal part of the colon. Following ATID, the stressing pathophysiological condition of the completely isolated tumor provoked a generalized cellular immune response, which led to the elimination of the devascularized tumor and distant lesions without causing sepsis. The patient did not experience any serious side-effects following the operation, and refused any adjuvant chemo- and/or radiotherapy. To date, the patient has no complaints and remains in good health after the ATID intervention, already more than 14 years. The present case study provides a typical demonstration of the clinical safety of ATID, and also indicates both the immunizing and the curative potential of the method.

Department of Human Pharmacology and Toxicology University of Veterinary and Pharmaceutical Sciences Brno 612 42 Brno Czech Republic

Former Scientist at Nestlé Research Center 1000 Lausanne 26 Switzerland

Institute for Neuropathic Pain 1056 SN Amsterdam The Netherlands

Navrátilová Bartoňková Hana Mediclinic a s 571 01 Moravská Třebová Czech Republic

Podřipská Nemocnice 413 01 Roudnice nad Labem Czech Republic

Zobrazit více v PubMed

Costi R, Leonardi F, Zanoni D, Violi V, Roncoroni L. Palliative care and end-stage colorectal cancer management: The surgeon meets the oncologist. World J Gastroenterol. 2014;20:7602–7621. doi: 10.3748/wjg.v20.i24.7602. PubMed DOI PMC

Kalina V, Kopsky DJ. Autologous tumor immunizing devascularization in cancer therapy. Med Hypotheses. 2016;89:72–78. doi: 10.1016/j.mehy.2016.02.004. PubMed DOI

Fortýn K, Hradecký J, Pazdera J, Klaudy J, Hruban V, Dvorak P, Matousek J, Tichý J, Kolín V. Experimental elimination of various intestinal segments by means of devascularization (devitalization) Z Exp Chir Transplant Kunstliche Organe. 1985;18:34–41. PubMed

Fortýn K, Hradecký J, Hruban V, Horák V, Dvorák P, Tichý J. Morphology of regressive changes in the kidney following experimental ischaemia. Int Urol Nephrol. 1987;19:9–19. doi: 10.1007/BF02549672. PubMed DOI

Fortýn K, Hruban V, Horák V. Treatment of malignant melanoma. Br J Surg. 1994;81:146–147. doi: 10.1002/bjs.1800810155. PubMed DOI

Horák V, Fortýn K, Hruban V, Klaudy J. Hereditary melanoblastoma in miniature pigs and its successful therapy by devitalization technique. Cell Mol Biol (Noisy-le-grand) 1999;45:1119–1129. PubMed

Friberg S, Mattson S. On the growth rates of human malignant tumors: Implications for medical decision making. J Surg Oncol. 1997;65:284–297. doi: 10.1002/(SICI)1096-9098(199708)65:4<284::AID-JSO11>3.0.CO;2-2. PubMed DOI

Choi SJ, Kim HS, Ahn SJ, Jeong YM, Choi HY. Evaluation of the growth pattern of carcinoma of colon and rectum by MDCT. Acta Radiol. 2013;54:487–492. doi: 10.1177/0284185113475923. PubMed DOI

Crowe SJ, Cushing H, Homans J. Effects of hypophyseal transplantation following total hypophysectomy in the canine. Quarterly Journal of Experimental Physiology. 1909;2:389–400. doi: 10.1113/expphysiol.1909.sp000049. DOI

Fortýn K, Hruban V, Hradecký J, Tichý J, Dvorák P, Horák V. Experimental devascularization (devitalization) of the rectum and sigmoideum. Z Exp Chir Transplant Kunstliche Organe. 1989;22:173–179. PubMed

Fortýn K, Hruban V, Hradecký J, Tichý J, Dvorák P, Horák V. The devitalization of the stomach by a devascularization technique. Acta Chir Hung. 1988;29:163–172. PubMed

Thompson RL, Leighton WE. The Results of Chronic Parathyroiditis as obtained by Ligation of the Parathyroid glandules in the Dog. J Med Res. 1908;19:121–134. PubMed PMC

Paulo DNS, Paulo ICAL, Loureiro ER, Pereira FEL, Grillo LSP, Jr, Silva ALD. Spleen viability after the simultaneous splenic artery and vein ligation in rats. Rev Col Bras Cir. 2005;32:32–35.

Troell A. On ligation of splenic vessels as a substitute for spelnectomy in blood diseases: Also a contribution to the pathology of the infarcts of the spleen. Ann Surg. 1916;63:88–105. doi: 10.1097/00000658-191601000-00007. PubMed DOI PMC

Griffiths J. Effects upon the Testes of Ligature of the Spermatic Artery, Spermatic Veins, and of both Artery and Veins. J Anat Physiol. 1895;30:81–105. PubMed PMC

Morgan TH. The effects of ligating the testes of Hen-feathered Cocks. Biological Bulletin. 1920;39:248–256. doi: 10.2307/1536490. DOI

Awasum C, Hassan AZ, Remi-Adewumi B, et al. Neovascularisation of the ovary post ligation of ovarian vessels in bitches. Sokoto J Vet Sci. 2008;7:7–9.

Papa M, Halperin Z, Rubinstein E, Orenstein A, Gafin S, Adar R. The effect of ischemia of the dog's colon on transmural migration of bacteria and endotoxin. J Surg Res. 1983;35:264–269. doi: 10.1016/S0022-4804(83)80013-4. PubMed DOI

Mayo CH. IV. Ligation of the thyroid vessels in cases of Hyperthyroidism. Ann Surg. 1909;50:1018–1024. doi: 10.1097/00000658-190912000-00004. PubMed DOI PMC

Horsley V. Further note on the possibility of curing Myxoedema. Br Med J. 1890;2:201–202. doi: 10.1136/bmj.2.1543.201. PubMed DOI PMC

Holt MP. Ligature of the renal vessels by the Transperitoneal method for the cure of persistent urinary renal fistula: With notes of one successful case. Med Chir Trans. 1907;90:469–478. PubMed PMC

Lo CC, Hsu PI, Lo GH, Tseng HH, Chen HC, Hsu PN, Lin CK, Chan HH, Tsai WL, Chen WC, et al. Endoscopic banding ligation can effectively resect hyperplastic polyps of stomach. World J Gastroenterol. 2003;9:2805–2808. doi: 10.3748/wjg.v9.i12.2805. PubMed DOI PMC

Quintero RA, Romero R, Reich H, Gonçalves L, Johnson MP, Carreño C, Evans MI. In utero percutaneous umbilical cord ligation in the management of complicated monochorionic multiple gestations. Ultrasound Obstet Gynecol. 1996;8:16–22. doi: 10.1046/j.1469-0705.1996.08010016.x. PubMed DOI

Sheu EG, Wakatsuki K, Younan G, Oakes SM, Moore FD., Jr Ischemic injury inhibits cancer metastasis by modulation of host adaptive immunity. J Am Col Surg. 2011;213:S137. (Suppl)

Kamijo A, Koshino T, Uesugi M, Nitto H, Saito T. Inhibition of lung metastasis of osteosarcoma cell line POS-1 transplanted into mice by thigh ligation. Cancer lett. 2002;188:213–219. doi: 10.1016/S0304-3835(02)00433-0. PubMed DOI

Parkins CS, Hill SA, Lonergan SJ, Horsman MR, Chadwick JA, Chaplin DJ. Ischaemia induced cell death in tumors: Importance of temperature and pH. Int J Radiat Oncol Biol Phys. 1994;29:499–503. doi: 10.1016/0360-3016(94)90445-6. PubMed DOI

Allen FM. Local asphyxia and tumors. Am J Surg. 1940;49:484–489. doi: 10.1016/S0002-9610(40)90587-6. DOI

Allen FM, Kaplan MM, Meranze DR, Gradess M. Treatment of spontaneous tumours by temporary local ligation. A comparative study in dogs. Bull World Health Organ. 1960;23:727–737. PubMed PMC

Veloso R, Pinho R, Rodrigues A, Pais T, Fernandes C, Carvalho J, Fraga J. Endoloop ligation (‘loop-and-let-go’) of a large ileal lipoma by balloon-assisted enteroscopy. Endoscopy. 2012;44(Suppl 2):E176. UCTN. PubMed

Melanoma-Bearing Libechov Minipig (MeLiM): The Unique Swine Model of Hereditary Metastatic Melanoma

Tumour devascularisation as a potential immunotherapeutic strategy