Neutrophil gelatinase-associated lipocalin production negatively correlates with HK-2 cell impairment: Evaluation of NGAL as a marker of toxicity in HK-2 cells
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27888128
DOI
10.1016/j.tiv.2016.11.012
PII: S0887-2333(16)30243-0
Knihovny.cz E-zdroje
- Klíčová slova
- Cell viability, HK-2 cells, In vitro nephrotoxicity, NGAL, Nephrotoxicity markers,
- MeSH
- akutní poškození ledvin chemicky indukované metabolismus MeSH
- biologické markery metabolismus MeSH
- buněčné linie MeSH
- cisplatina toxicita MeSH
- gentamiciny toxicita MeSH
- kadmium toxicita MeSH
- lidé MeSH
- lipokalin-2 genetika metabolismus MeSH
- paracetamol toxicita MeSH
- rtuť toxicita MeSH
- terc-butylhydroperoxid toxicita MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biologické markery MeSH
- cisplatina MeSH
- gentamiciny MeSH
- kadmium MeSH
- LCN2 protein, human MeSH Prohlížeč
- lipokalin-2 MeSH
- paracetamol MeSH
- rtuť MeSH
- terc-butylhydroperoxid MeSH
Neutrophil gelatinase-associated lipocalin is an extracellular protein produced mostly in kidney. Recently, it has become a promising biomarker of renal damage in vivo. On the other hand, the validation of NGAL as a biomarker for nephrotoxicity estimation in vitro has not been characterized in detail yet. Since the HK-2 cells are frequently used human kidney cell line, we aimed to characterize the production of NGAL in these cells and to evaluate NGAL as a possible marker of cell impairment. We used heavy metals (mercury, cadmium), peroxide, drugs (acetaminophen, gentamicin) and cisplatin to mimic nephrotoxicity. HK-2 cells were incubated with selected compounds for 1-24h and cell viability was measured together with extracellular NGAL production. We proved that HK-2 cells possess a capacity to produce NGAL in amount of 2pg/ml/h. We found a change in cell viability after 24h incubation with all tested toxic compounds. The largest decrease of the viability was detected in mercury, acetaminophen, cisplatin and gentamicin. Unexpectedly, we found also a significant decrease in NGAL production in HK-2 cells treated with these toxins for 24h: to 11±5%, 54±5%, 57±6% and 76±9% respectively, compared with controls (=100%). Our results were followed with qPCR analysis when we found no significant increase in LCN2 gene expression after 24h incubation. We conclude that extracellular NGAL production negatively correlates with HK-2 cell impairment.
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