Antibody-free detection of infectious bacteria using quantum dots-based barcode assay
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27894780
DOI
10.1016/j.jpba.2016.10.025
PII: S0731-7085(16)30995-5
Knihovny.cz E-resources
- Keywords
- Barcode, Klebsiella pneumoniae, Magnetic particles, Methicillin-resistant Staphylococcus aureus, Quantum dots, Staphylococcus aureus,
- MeSH
- Klebsiella pneumoniae chemistry genetics MeSH
- Quantum Dots chemistry MeSH
- Humans MeSH
- Microbial Sensitivity Tests methods MeSH
- Staphylococcus aureus chemistry genetics MeSH
- DNA Barcoding, Taxonomic methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment. The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detect as low concentrations of bacteria as 102 CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).
References provided by Crossref.org
Magnetic Nanoparticles: From Design and Synthesis to Real World Applications
