Fast and sensitive analysis of beta blockers by ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27925253
DOI
10.1002/bmc.3911
Knihovny.cz E-resources
- Keywords
- beta blockers, forensic toxicological screening, liquid chromatography, mass spectrometry, therapeutic drug monitoring,
- MeSH
- Adrenergic beta-Antagonists blood MeSH
- Mass Spectrometry methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Limit of Detection MeSH
- Reference Standards MeSH
- Reproducibility of Results MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adrenergic beta-Antagonists MeSH
This paper presents a method for the determination of acebutolol, betaxolol, bisoprolol, metoprolol, nebivolol and sotalol in human serum by liquid-liquid extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. After liquid-liquid extraction, beta blockers were separated on a reverse-phase analytical column (Acclaim RS 120; 100 × 2.1 mm, 2.2 μm). The total run time was 6 min for each sample. Linearity, limit of detection, limit of quantification, matrix effects, specificity, precision, accuracy, recovery and sample stability were evaluated. The method was successfully applied to the therapeutic drug monitoring of 108 patients with hypertension. This method was also used for determination of beta blockers in 33 intoxicated patients.
Department of Forensic Medicine and Medical Law University Hospital Olomouc Olomouc Czech Republic
Faculty of Medicine and Dentistry Palacky University Olomouc Czech Republic
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