Fast and sensitive analysis of beta blockers by ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27925253
DOI
10.1002/bmc.3911
Knihovny.cz E-zdroje
- Klíčová slova
- beta blockers, forensic toxicological screening, liquid chromatography, mass spectrometry, therapeutic drug monitoring,
- MeSH
- beta blokátory krev MeSH
- hmotnostní spektrometrie metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- limita detekce MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beta blokátory MeSH
This paper presents a method for the determination of acebutolol, betaxolol, bisoprolol, metoprolol, nebivolol and sotalol in human serum by liquid-liquid extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. After liquid-liquid extraction, beta blockers were separated on a reverse-phase analytical column (Acclaim RS 120; 100 × 2.1 mm, 2.2 μm). The total run time was 6 min for each sample. Linearity, limit of detection, limit of quantification, matrix effects, specificity, precision, accuracy, recovery and sample stability were evaluated. The method was successfully applied to the therapeutic drug monitoring of 108 patients with hypertension. This method was also used for determination of beta blockers in 33 intoxicated patients.
Department of Forensic Medicine and Medical Law University Hospital Olomouc Olomouc Czech Republic
Faculty of Medicine and Dentistry Palacky University Olomouc Czech Republic
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