IGRA metody v rutinním provozu - QuantiFERON®-TB Gold nebo T-SPOT®.TB?
[IGRA methods in the routine operation - QuantiFERON®-TB Gold or T-SPOT.TB?]
Language Czech Country Czech Republic Media print
Document type Journal Article
PubMed
28078903
PII: 60021
- Keywords
- IGRA - Mycobacterium tuberculosis - Quantiferon - T-SPOT®.TB.,
- MeSH
- Adult MeSH
- Immunoassay methods MeSH
- Interferon-gamma blood MeSH
- Humans MeSH
- Predictive Value of Tests MeSH
- Reagent Kits, Diagnostic MeSH
- Tuberculin Test methods MeSH
- Tuberculosis blood diagnosis MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Interferon-gamma MeSH
- Reagent Kits, Diagnostic MeSH
For indirect diagnosis of tuberculosis, two commercial IGRA (Interferon Gamma Release Assay) assays are available - primal QuantiFERON®-TB Gold test, new version QuantiFERON®-TB Gold Plus test (four tube, differentiation in activity CD4+ a CD8+) and T-SPOT®.TB test. Both methods are based on the same principle, but their workflows are different. In this article, both assays are compared on the collection of 284 patients. Inter-rate agreement measure showed 81.3% consistency and Cohens kappa index was calculated as 0.72. In case of discrepancy between IGRA and other methods (clinical aspects, X-ray diagnostic, etc.), results should be confirmed by second IGRA assay for correct interpretation.
