Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
16-31593A
Grant of Ministry of Health, CZ
201404
Internal grant of the Centre for Cardiovascular Surgery and Transplantation Brno, CZ
PubMed
28365878
DOI
10.1007/s12223-017-0526-7
PII: 10.1007/s12223-017-0526-7
Knihovny.cz E-zdroje
- Klíčová slova
- Antibiotic Susceptibility, Antimicrobial Susceptibility Pattern, Common Genotype, Meropenem, Unique Genotype,
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence MeSH
- biofilmy * MeSH
- dospělí MeSH
- genotyp MeSH
- jednotky intenzivní péče statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mladý dospělý MeSH
- pseudomonádové infekce mikrobiologie MeSH
- Pseudomonas aeruginosa účinky léků genetika izolace a purifikace fyziologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- technika náhodné amplifikace polymorfní DNA MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed. RAPD typing in strains with the same serotype revealed different genotypes and, on the contrary, most strains with a different serotype displayed the same amplification pattern. The resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients. A higher degree of antibiotic resistance and stronger biofilm production in common genotypes compared to rare ones and genetic homogeneity of the most resistant strains indicated the role of antibiotic pressure in acquiring resistant and more virulent strains in our hospital. In conclusion, genetic characterisation of P. aeruginosa strains using RAPD method was shown to be more accurate in epidemiological analyses than phenotyping.
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