BACKGROUND: Tropical rainforests are frequently limited by soil nutrient availability. However, the response of the metabolic phenotypic plasticity of trees to an increase of soil nutrient availabilities is poorly understood. We expected that increases in the ability of a nutrient that limits some plant processes should be detected by corresponding changes in plant metabolome profile related to such processes. METHODOLOGY/PRINCIPAL FINDINGS: We studied the foliar metabolome of saplings of three abundant tree species in a 15 year field NPK fertilization experiment in a Panamanian rainforest. The largest differences were among species and explained 75% of overall metabolome variation. The saplings of the large canopy species, Tetragastris panamensis, had the lowest concentrations of all identified amino acids and the highest concentrations of most identified secondary compounds. The saplings of the "mid canopy" species, Alseis blackiana, had the highest concentrations of amino acids coming from the biosynthesis pathways of glycerate-3P, oxaloacetate and α-ketoglutarate, and the saplings of the low canopy species, Heisteria concinna, had the highest concentrations of amino acids coming from the pyruvate synthesis pathways. CONCLUSIONS/SIGNIFICANCE: The changes in metabolome provided strong evidence that different nutrients limit different species in different ways. With increasing P availability, the two canopy species shifted their metabolome towards larger investment in protection mechanisms, whereas with increasing N availability, the sub-canopy species increased its primary metabolism. The results highlighted the proportional distinct use of different nutrients by different species and the resulting different metabolome profiles in this high diversity community are consistent with the ecological niche theory.

CREAF Cerdanyola del Vallès Catalonia Spain

CSIC Global Ecology Unit CREAF CEAB CSIC UAB Cerdanyola del Vallès Catalonia Spain

Global Change Research Institute The Czech Academy of Sciences Brno Czech Republic

Servei de Ressonància Magnètica Nuclear Faculty of Sciences and Biosciences Universitat Autònoma de Barcelona Bellaterra Barcelona Catalonia Spain

Smithsonian Tropical Research Institute Apartado Balboa Republic of Panama

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Tree Species and Epiphyte Taxa Determine the "Metabolomic niche" of Canopy Suspended Soils in a Species-Rich Lowland Tropical Rainforest

Different "metabolomic niches" of the highly diverse tree species of the French Guiana rainforests

Root exudate metabolomes change under drought and show limited capacity for recovery