Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus)
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28534230
DOI
10.1007/s12223-017-0533-8
PII: 10.1007/s12223-017-0533-8
Knihovny.cz E-zdroje
- MeSH
- absces mozku mikrobiologie veterinární MeSH
- Actinomycetaceae klasifikace genetika izolace a purifikace fyziologie MeSH
- bakteriální proteiny genetika metabolismus MeSH
- faktory virulence genetika metabolismus MeSH
- fylogeneze MeSH
- infekce bakteriemi řádu Actinomycetales mikrobiologie veterinární MeSH
- vysoká zvěř MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- faktory virulence MeSH
The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.
Department of Veterinary Public Health College of Veterinary Medicine University of Mosul Mosul Iraq
Entwicklung Bioanalyse Bruker Daltonik GmbH Fahrenheitstraße 4 28359 Bremen Germany
Landesbetrieb Hessisches Landeslabor Schubertstraße 60 35392 Giessen Germany
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