Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26883140
DOI
10.1007/s12223-016-0450-2
PII: 10.1007/s12223-016-0450-2
Knihovny.cz E-zdroje
- MeSH
- Arcanobacterium genetika izolace a purifikace fyziologie MeSH
- bakteriální proteiny genetika MeSH
- diagnostické techniky molekulární metody MeSH
- DNA bakterií chemie genetika MeSH
- fylogeneze MeSH
- infekce bakteriemi řádu Actinomycetales mikrobiologie veterinární MeSH
- mastitida skotu mikrobiologie MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- mléko mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- skot MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- techniky typizace bakterií metody MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- mezerníky ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.
Department of Veterinary Public Health College of Veterinary Medicine University of Mosul Mosul Iraq
Entwicklung Bioanalyse Bruker Daltonik GmbH Fahrenheitstraße 4 28359 Bremen Germany
Tiergesundheitsdienst Bayern e 5 Eutergesundheitsdienst Senator Gerauer Straße 23 85586 Grub Germany
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