This study investigated the influence of aeration and minimal medium conditions on antioxidant and antibacterial activities of 21 probiotic Lactobacillus strains isolated from dairy products. The probiotic potential of the isolates was evaluated by pH and bile tolerance. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to confirm the phenotypic identification of isolates. Antioxidant producer isolates were screened by resistance to reactive oxygen species (ROS). The antioxidant and antibacterial activities of extracellular materials after 48 h fermentation with antioxidative strains were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and broth microdilution assays, respectively. The results indicate that the antioxidant capacity of supernatants was increased by using of both minimal medium and agitation. The antibacterial activity was increased in minimal medium, but there has nearly no change in the antibacterial properties by using both agitation and minimal medium. The maximum antibacterial activity was observed during mid-exponential phase until the beginning of the early-stationary phase, but the maximum antioxidant activity was detected at the stationary growth phase. There is a significant relationship between antioxidant and antibacterial activities of the cell-free probiotic extracts, and their production rates are closely related to the fermentation type. The bioactive materials from probiotics could be extracted in a large amount at an appropriate time under a suitable condition.

Department of Biology Medicinal Plants and Drugs Research Institute Shahid Beheshti University Tehran Iran

Department of Microbiology Faculty of Biological Sciences Alzahra University Tehran Iran

Department of Phytochemistry Medicinal Plants and Drugs Research Institute Shahid Beheshti University Tehran Iran

Molecular Biology Unit Pasteur Institute of Iran Pasteur Ave Tehran 13164 Iran

Zobrazit více v PubMed

Lett Appl Microbiol. 2010 Jan;50(1):57-64 PubMed

Oral Microbiol Immunol. 2008 Apr;23(2):139-47 PubMed

Anaerobe. 2014 Jun;27:22-6 PubMed

Meat Sci. 2014 Dec;98(4):575-84 PubMed

Molecules. 2012 Feb 10;17(2):1797-808 PubMed

Am J Clin Nutr. 2001 Feb;73(2 Suppl):386S-392S PubMed

J Biotechnol. 2002 May 23;95(3):225-35 PubMed

Clin Microbiol Rev. 2003 Oct;16(4):658-72 PubMed

Jundishapur J Nat Pharm Prod. 2013 Aug;8(3):131-7 PubMed

Mol Pharm. 2008 Mar-Apr;5(2):167-90 PubMed

Antonie Van Leeuwenhoek. 2001 Jan;79(1):1-6 PubMed

J Appl Microbiol. 2003;94(3):403-12 PubMed

Pharmacogn Rev. 2010 Jul;4(8):118-26 PubMed

Appl Environ Microbiol. 1999 Aug;65(8):3418-26 PubMed

Lett Appl Microbiol. 2008 Oct;47(4):269-74 PubMed

Int J Food Microbiol. 2002 Feb 5;72(3):215-24 PubMed

Int J Food Microbiol. 2003 Feb 25;81(1):41-52 PubMed

Chembiochem. 2009 Mar 2;10(4):625-33 PubMed