Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28688831
DOI
10.1016/j.autneu.2017.06.003
PII: S1566-0702(16)30259-4
Knihovny.cz E-resources
- Keywords
- Endothelial cells, Guanethidine, Osmotic mini pump, Sympathetic innervation,
- MeSH
- Human Umbilical Vein Endothelial Cells drug effects metabolism pathology MeSH
- Femur drug effects innervation metabolism pathology MeSH
- Fluorescent Antibody Technique MeSH
- Guanethidine pharmacology MeSH
- Bone Marrow drug effects innervation metabolism MeSH
- Humans MeSH
- Mesenchymal Stem Cells drug effects metabolism pathology MeSH
- Models, Animal MeSH
- Rats, Wistar MeSH
- Flow Cytometry MeSH
- Sympathectomy, Chemical MeSH
- Sympathetic Nervous System drug effects metabolism pathology MeSH
- Sympatholytics pharmacology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Guanethidine MeSH
- Sympatholytics MeSH
Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naïve femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin+/SDF1+ mesenchymal stem cells and c-Kit+/CD90+ hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.
Department of Anatomy Faculty of Medicine Masaryk University Czech Republic
Department of Histology and Embryology Faculty of Medicine Masaryk University Czech Republic
Department of Internal Medicine Hematology and Oncology University Hospital Brno Czech Republic
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