The control of the surface properties is an important issue for applicability of polymer membranes interacting with cells. In this work, the influence of surface roughness and stiffness of two polymer membranes on viability and mechanical properties of keratinocytes was studied. Terpolimer polyglicolide, polycaprolactone and polylactide, (PGA-PCL-PLA) and copolymer polycaprolactone, polyglicolide (PGA-PCL) substrates were used for membranes fabrication. Surface modification - the hydrolysis of the obtained membranes was carried out. The analysis of membranes' surface properties revealed that RMS surface roughness and roughness factor of PGA-PCL-PLA membrane decreased after hydrolysis while its stiffness increased. In contrast, the PGA-PCL membrane stiffness was only slightly affected by NaOH treatment. Immortalized human keratinocytes (HaCaT) were grown under standard conditions on the surface of the studied membranes and characterized by means of atomic force microscopy and fluorescence microcopy. The results showed the substrate-dependent effect on cells' properties.

Centre of Polymer and Carbon Materials Polish Academy of Sciences M Curie Skłodowskiej 34 41 819 Zabrze Poland

Czech Technical University Prague Faculty of Mechanical Engineering Technicka 4 16607 Praha Czechia

Faculty of Mathematics and Information Science Warsaw University of Technology Koszykowa 75 00 662 Warsaw Poland

Institute of Biotechnology Faculty of Chemistry Warsaw University of Technology Noakowskiego 3 00 664 Warsaw Poland

Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Trojdena 4 109 02 Warsaw Poland

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