Alloscardovia venturai sp. nov., a fructose 6-phosphate phosphoketolase-positive species isolated from the oral cavity of a guinea-pig (Cavia aperea f. porcellus)
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
- MeSH
- Actinobacteria classification genetics isolation & purification MeSH
- Aldehyde-Lyases metabolism MeSH
- Genes, Bacterial MeSH
- DNA, Bacterial genetics MeSH
- Fructose MeSH
- Phylogeny * MeSH
- Fatty Acids chemistry MeSH
- Guinea Pigs microbiology MeSH
- Peptidoglycan chemistry MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Mouth microbiology MeSH
- Base Composition MeSH
- Animals MeSH
- Check Tag
- Guinea Pigs microbiology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Aldehyde-Lyases MeSH
- DNA, Bacterial MeSH
- fructose-6-phosphate phosphoketolase MeSH Browser
- Fructose MeSH
- Fatty Acids MeSH
- Peptidoglycan MeSH
- RNA, Ribosomal, 16S MeSH
A slightly irregular, short rod-shaped bacterial strain, MOZIV/2T, showing activity of fructose 6-phosphate phosphoketolase was isolated from the oral cavity of a home-bred guinea-pig. Based on comparative 16S rRNA gene sequence analyses, its closest relatives were Alloscardovia omnicolens DSM 21503T and Alloscardovia criceti DSM 17774T with 96.0 and 95.6 % pairwise similarities, respectively. Completeness of the compared sequences was 97.3 and 96.9 %, respectively. Growth was found only under anaerobic conditions. Activities of α- and β-gluco(galacto)sidases were detected in strain MOZIV/2T, which is characteristic for almost all members of the family Bifidobacteriaceae. Sequencing of other molecular markers (fusA, gyrB and xfp) revealed low gene sequence similarities to A. omnicolens DSM 21503T ranging from 72.7 to 87.5 %. Strain MOZIV/2T differed from other species within the genus Alloscardovia by the presence of C18 : 1ω9t. In addition, much higher proportions of C8 : 0, C11 : 0, C12 : 0, C14 : 1, C16 : 1 and C17 : 0 fatty acids were found in cells of strain MOZIV/2T. The peptidoglycan structure was of type A4α [l-Lys(l-Orn)-d-Asp], which is consistent with its classification within the genus Alloscardovia. The DNA G+C content (45.8 mol%) was lower than those found in other alloscardovia. Phylogenetic studies and evaluation of phenotypic characteristics including the results of biochemical, physiological and chemotaxonomic analyses confirmed the novel species status for strain MOZIV/2T, for which the name Alloscardovia venturai sp. nov. is proposed. The type strain is MOZIV/2T (=DSM 100237T=CCM 8604T=LMG 28781T).
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