Glutamine synthetase type I (glnAI) represents a rewarding molecular marker in the classification of bifidobacteria and related genera
Language English Country United States Media print-electronic
Document type Journal Article
Grant support
CZ.02.1.01/0.0/0.0/15_003/0000460
the project of excellence
QJ1510338
Czech National Agency for Agricultural Research
16-27449A
Czech Health Research Council
PubMed
31069634
DOI
10.1007/s12223-019-00716-0
PII: 10.1007/s12223-019-00716-0
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial MeSH
- Bifidobacterium classification enzymology MeSH
- DNA, Bacterial genetics MeSH
- DNA Primers MeSH
- Phylogeny * MeSH
- Genetic Markers MeSH
- Genotype MeSH
- Glutamate-Ammonia Ligase genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA Primers MeSH
- Genetic Markers MeSH
- glutamine synthetase I MeSH Browser
- Glutamate-Ammonia Ligase MeSH
- RNA, Ribosomal, 16S MeSH
The family Bifidobacteriaceae constitutes an important phylogenetic group that particularly includes bifidobacterial taxa demonstrating proven or debated positive effects on host health. The increasingly widespread application of probiotic cultures in the twenty-first century requires detailed classification to the level of particular strains. This study aimed to apply the glutamine synthetase class I (glnAI) gene region (717 bp representing approximately 50% of the entire gene sequence) using specific PCR primers for the classification, typing, and phylogenetic analysis of bifidobacteria and closely related scardovial genera. In the family Bifidobacteriaceae, this is the first report on the use of this gene for such purposes. To achieve high-value results, almost all valid Bifidobacteriaceae type strains (75) and 15 strains isolated from various environments were evaluated. The threshold value of the glnAI gene identity among Bifidobacterium species (86.9%) was comparable to that of other phylogenetic/identification markers proposed for bifidobacteria and was much lower compared to the 16S rRNA gene. Further statistical and phylogenetic analyses suggest that the glnAI gene can be applied as a novel genetic marker in the classification, genotyping, and phylogenetic analysis of isolates belonging to the family Bifidobacteriaceae.
See more in PubMed
Syst Biol. 2007 Aug;56(4):564-77 PubMed
Res Microbiol. 2010 Mar;161(2):82-90 PubMed
Int J Syst Evol Microbiol. 2017 Aug;67(8):2842-2847 PubMed
Int J Syst Evol Microbiol. 2006 Dec;56(Pt 12):2783-92 PubMed
Syst Appl Microbiol. 2010 Nov;33(7):359-66 PubMed
Int J Syst Evol Microbiol. 2017 Oct;67(10):3987-3995 PubMed
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4439-46 PubMed
Infect Genet Evol. 2013 Dec;20:188-96 PubMed
Bioinformatics. 2010 Oct 1;26(19):2462-3 PubMed
J Microbiol. 2018 Oct;56(10):713-721 PubMed
Int J Syst Evol Microbiol. 2018 Feb;68(2):575-581 PubMed
Microbiologyopen. 2018 Aug;7(4):e00579 PubMed
Benef Microbes. 2014 Dec;5(4):377-88 PubMed
Front Microbiol. 2018 Aug 22;9:2007 PubMed
BMC Genomics. 2017 Aug 1;18(1):568 PubMed
PLoS One. 2011 Mar 31;6(3):e14792 PubMed
BMC Bioinformatics. 2003 Sep 11;4:41 PubMed
Int J Syst Evol Microbiol. 2001 Sep;51(Pt 5):1633-8 PubMed
Appl Environ Microbiol. 2003 Nov;69(11):6908-22 PubMed
BMC Evol Biol. 2009 Feb 26;9:48 PubMed
FEMS Microbiol Lett. 2012 Sep;334(1):1-15 PubMed
Appl Environ Microbiol. 2014 Oct;80(20):6383-94 PubMed
Int J Syst Evol Microbiol. 2014 May 27;64(9):2932-2938 PubMed
Mol Biol Evol. 2000 Feb;17(2):309-19 PubMed
Biochim Biophys Acta. 2000 Mar 7;1477(1-2):122-45 PubMed
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1434-51 PubMed
Int J Syst Evol Microbiol. 2017 Jul;67(7):2403-2411 PubMed
Folia Microbiol (Praha). 2010 Jul;55(4):336-9 PubMed
Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1442-6 PubMed
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4350-3 PubMed
Int J Syst Evol Microbiol. 2018 Jan;68(1):461-466 PubMed
Syst Appl Microbiol. 2015 Jun;38(4):237-45 PubMed
Microbiome. 2016 May 03;4(1):18 PubMed
Nat Rev Gastroenterol Hepatol. 2014 Aug;11(8):506-14 PubMed
Int J Syst Evol Microbiol. 2017 Jul;67(7):2349-2356 PubMed
