Reclassification of Bifidobacterium stercoris Kim et al. 2010 as a later heterotypic synonym of Bifidobacterium adolescentis
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Aldehyde-Lyases genetics MeSH
- Bifidobacterium classification genetics MeSH
- DNA, Bacterial genetics MeSH
- DNA Gyrase genetics MeSH
- DNA-Directed RNA Polymerases genetics MeSH
- Peptide Elongation Factor G genetics MeSH
- Phenotype MeSH
- Phylogeny * MeSH
- Nucleic Acid Hybridization MeSH
- Molecular Sequence Data MeSH
- Multilocus Sequence Typing MeSH
- Ribosomal Proteins genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Aldehyde-Lyases MeSH
- beta' subunit of RNA polymerase MeSH Browser
- DNA, Bacterial MeSH
- DNA Gyrase MeSH
- DNA-Directed RNA Polymerases MeSH
- Peptide Elongation Factor G MeSH
- fructose-6-phosphate phosphoketolase MeSH Browser
- ribosomal protein L2 MeSH Browser
- Ribosomal Proteins MeSH
- RNA, Ribosomal, 16S MeSH
The taxonomic position of Bifidobacterium stercoris Eg1(T) ( = JCM 15918(T)) based on comparative 16S rRNA gene and hsp60 sequence analyses was found to be controversial, as the strain showed high similarity to the type strain of Bifidobacterium adolescentis, CCUG 18363(T). Therefore, the relationship between the two species was investigated by a taxonomic study that included, in addition to re-evaluation of the 16S rRNA gene sequence, determination of DNA-DNA binding and multilocus sequence analysis (MLSA) of housekeeping genes encoding the DNA-directed RNA polymerase B subunit (rpoC), putative xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp), elongation factor EF-G (fusA), 50S ribosomal protein L2 (rplB) and DNA gyrase B subunit (gyrB). Comparative 16S rRNA gene sequence analysis showed relatively high similarity (98.9 %) between B. stercoris KCTC 5756(T) and B. adolescentis ATCC 15703(T). MLSA revealed close relatedness between B. stercoris KCTC 5756(T) and B. adolescentis CCUG 18363(T), with 99.3-100 % similarity between the rpoC, xfp, fusA, rplB and gyrB gene sequences. In addition, relatively high dnaJ1 gene sequence similarity of 97.7 % was found between the strains. Similar phenotypes and a high DNA-DNA binding value (78.9 %) confirmed that B. stercoris and B. adolescentis are synonymous. Based on these results, it is proposed that the species Bifidobacterium stercoris Kim et al. 2010 should be reclassified as a later heterotypic synonym of Bifidobacterium adolescentis Reuter 1963 (Approved Lists 1980).
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GENBANK
JQ363622, JQ363623, JQ363628, JQ363629, JQ363638, JQ363639, JQ363655, JQ363656, JQ363659, JQ363660, JQ363666, JQ363667, KF147852
