Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol-gel modified inner capillary wall
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28851524
DOI
10.1016/j.chroma.2017.08.014
PII: S0021-9673(17)31175-5
Knihovny.cz E-resources
- Keywords
- Capillary electrochromatography (CEC), Nucleo-compounds, Oligopeptides, Open-tubular capillary electrochromatography (OT-CEC), Porphyrin, Scanning electron microscopy (SEM), Sol–gel methods,
- MeSH
- Chemistry Techniques, Analytical instrumentation methods MeSH
- Capillary Electrochromatography * MeSH
- Microscopy, Electron, Scanning MeSH
- Nucleosides isolation & purification MeSH
- Nucleotides isolation & purification MeSH
- Oligopeptides isolation & purification MeSH
- Phase Transition MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Nucleosides MeSH
- Nucleotides MeSH
- Oligopeptides MeSH
The aim of this article is to study the modification of an inner capillary wall with sol-gel coating (pure silica sol-gel or silica sol-gel containing porphyrin-brucine conjugate) and determine its influence on the separation process using capillary electrophoresis/electrochromatography method. After modification of the inner capillary surface the separation of analytes was performed using two different phosphate buffers (pH 2.5 and 9.0) and finally the changes in electrophoretic mobilities of various samples were calculated. To confirm that the modification of the inner capillary surface was successful, the parts of the inner surfaces of capillaries were observed using scanning electron microscopy. The analytes used as testing samples were oligopeptides, nucleosides, nucleobases and finally nucleotides.
Department of Analytical Chemistry Institute of Chemical Technology Prague Czech Republic
Institute of Physiology Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
