AIM: Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. Methodology & results: An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20-200 ng ml-1 in rat and macaque plasma samples. CONCLUSION: A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.

Department of Analytical Chemistry University of Chemistry and Technology Prague Technická 5 16628 Prague Czech Republic

Department of Biochemistry and Molecular Biology Institute of Organic Chemistry and Biochemistry AS CR Flemingovo náměstí 2 16610 Prague Czech Republic

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Lipidization as a tool toward peptide therapeutics

Lipidized PrRP Analog Exhibits Strong Anti-Obesity and Antidiabetic Properties in Old WKY Rats with Obesity and Glucose Intolerance