Mucilaginibacter terrae sp. nov., isolated from Antarctic soil
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
- Keywords
- Antarctica, James ross island, Mucilaginibacter terrae sp. nov., soil, taxonomy,
- MeSH
- Bacteroidetes classification genetics isolation & purification MeSH
- DNA, Bacterial genetics MeSH
- Phosphatidylethanolamines chemistry MeSH
- Phosphatidylserines chemistry MeSH
- Phylogeny * MeSH
- Fatty Acids chemistry MeSH
- Pigmentation MeSH
- Soil Microbiology * MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Spermidine analogs & derivatives chemistry MeSH
- Bacterial Typing Techniques MeSH
- Vitamin K 2 analogs & derivatives chemistry MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Antarctic Regions MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Phosphatidylethanolamines MeSH
- Phosphatidylserines MeSH
- Fatty Acids MeSH
- menaquinone 7 MeSH Browser
- phosphatidylethanolamine MeSH Browser
- RNA, Ribosomal, 16S MeSH
- Spermidine MeSH
- sym-homospermidine MeSH Browser
- Vitamin K 2 MeSH
A bacterial strain designated CCM 8645T was isolated from a soil sample collected nearby a mummified seal carcass in the northern part of James Ross Island, Antarctica. The cells were short rods, Gram-stain-negative, non-motile, catalase and oxidase positive, and produced a red-pink pigment on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, extensive biotyping using conventional tests and commercial identification kits and chemotaxonomic analyses were applied to clarify its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene placed strain CCM 8645T in the genus Mucilaginibacter with the closest relative being Mucilaginibacter daejeonensis Jip 10T, exhibiting 96.5 % 16S rRNA pairwise similarity which was clearly below the 97 % threshold value recommended for species demarcation. The major components in fatty acid profiles were Summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C15 : 0 iso and C17 : 0 iso 3OH. The cellular quinone content was exclusively menaquinone MK-7. The major polyamine was sym-homospermidine and predominant polar lipids were phosphatidylethanolamine and phosphatidylserine. Based on presented results, we propose a novel species for which the name Mucilaginibacter terrae sp. nov. is suggested, with the type strain CCM 8645T (=LMG 29437T).
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