Urinary intermediates of tryptophan as indicators of the gut microbial metabolism
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28916042
DOI
10.1016/j.aca.2017.08.022
PII: S0003-2670(17)30959-5
Knihovny.cz E-resources
- Keywords
- Gut microbiome, Methyl indol-3-propionate, Methyl indole-3-acetate, N-acetyltryptophan, Tryptophan metabolism, Urinary metabolome,
- MeSH
- Indoles urine MeSH
- Indoleacetic Acids urine MeSH
- Humans MeSH
- Gastrointestinal Microbiome * MeSH
- Tandem Mass Spectrometry MeSH
- Tryptophan metabolism urine MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- indoleacetic acid MeSH Browser
- indolepropionic acid MeSH Browser
- Indoles MeSH
- Indoleacetic Acids MeSH
- Tryptophan MeSH
While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.
Department of Clinical Hematology University Hospital Brno Brno Czech Republic
Research Centre for Toxic Compounds in the Environment Brno Czech Republic
References provided by Crossref.org
Urinary Metabolomic Profile in Children with Autism Spectrum Disorder
