Disruption of dopamine D1/D2 receptor complex is involved in the function of haloperidol in cardiac H9c2 cells
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
29054453
DOI
10.1016/j.lfs.2017.10.026
PII: S0024-3205(17)30540-4
Knihovny.cz E-resources
- Keywords
- D1R/D2R heterodimeric complex, Dopamine D2 receptor, H9c2 cell line, Haloperidol,
- MeSH
- Dopamine D2 Receptor Antagonists pharmacology MeSH
- Dopamine Antagonists pharmacology MeSH
- Cell Line MeSH
- Haloperidol pharmacology MeSH
- Rats, Wistar MeSH
- Receptors, Dopamine D1 genetics metabolism MeSH
- Receptors, Dopamine D2 genetics metabolism MeSH
- Gene Expression Regulation drug effects MeSH
- Signal Transduction drug effects MeSH
- Heart drug effects MeSH
- Heart Rate drug effects MeSH
- Protein Binding drug effects MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Dopamine D2 Receptor Antagonists MeSH
- Dopamine Antagonists MeSH
- Haloperidol MeSH
- Receptors, Dopamine D1 MeSH
- Receptors, Dopamine D2 MeSH
AIMS: Haloperidol is an antipsychotic agent and acts as dopamine D2 receptor (D2R) antagonist, as a prototypical ligand of sigma1 receptors (Sig1R) and it increases expression of type 1 IP3 receptors (IP3R1). However, precise mechanism of haloperidol action on cardiomyocytes through dopaminergic signaling was not described yet. This study investigated a role of dopamine receptors in haloperidol-induced increase in IP3R1 and Sig1R, and compared physiological effect of melperone and haloperidol on basic heart parameters in rats. MATERIALS AND METHODS: We used differentiated NG-108 cells and H9c2 cells. Gene expression, Western blot and immunofluorescence were used to evaluate haloperidol-induced differences; proximity ligation assay (PLA) and immunoprecipitation to determine interactions of D1/D2 receptors. To evaluate cardiac parameters, Wistar albino male rats were used. KEY FINDINGS: We have shown that antagonism of D2R with either haloperidol or melperone results in upregulation of both, IP3R1 and Sig1R, which is associated with increased D2R, but reduced D1R expression. Immunofluorescence, immunoprecipitation and PLA support formation of heteromeric D1/D2 complexes in H9c2 cells. Treatment with haloperidol (but not melperone) caused decrease in systolic and diastolic blood pressure and significant increase in heart rate. SIGNIFICANCE: Because D1R/D2R complexes can engage Gq-like signaling in other experimental systems, these results are consistent with the possibility that disruption of D1R/D2R complex in H9c2 cells might cause a decrease in IP3R1 activity, which in turn may account for the increase expression of IP3R and Sig1R. D2R is probably not responsible for changes in cardiac parameters, since melperone did not have any effect.
Department of Physiology Faculty of Medicine Masaryk University Brno Czech Republic
Institute of Virology Biomedical Research Center Slovak Academy of Sciences Bratislava Slovakia
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