Simultaneous analysis of carbohydrates, polyols and amines in urine samples using chemical ionization gas chromatography with tandem mass spectrometry
Language English Country Germany Media print-electronic
Document type Journal Article
- Keywords
- chemical ionization, direct silylation of urine, galactosemia, gas chromatography, tandem mass spectrometry,
- MeSH
- Algorithms MeSH
- Amines urine MeSH
- Urinalysis MeSH
- Adult MeSH
- Galactosemias urine MeSH
- Calibration MeSH
- Humans MeSH
- Limit of Detection MeSH
- Infant, Newborn MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Polymers analysis MeSH
- Reproducibility of Results MeSH
- Carbohydrates urine MeSH
- Tandem Mass Spectrometry MeSH
- Trimethylsilyl Compounds analysis MeSH
- Freezing MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amines MeSH
- N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide MeSH Browser
- Polymers MeSH
- polyol MeSH Browser
- Carbohydrates MeSH
- Trimethylsilyl Compounds MeSH
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL-1 for mannitol to 4.7 mg/L for glucose.
Axxence Slovakia s r o Bratislava Slovakia
Department of Laboratory Medicine University Children´s Hospital Bratislava Slovakia
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