A simplified globally affordable experimental setup for monitoring DNA diagnosis by a QD-based technique
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article
Grant support
-------
Research Council of University of Tehran
PubMed
29116587
DOI
10.1007/s12223-017-0554-3
PII: 10.1007/s12223-017-0554-3
Knihovny.cz E-resources
- MeSH
- Typhoid Fever diagnosis microbiology MeSH
- Molecular Diagnostic Techniques instrumentation methods MeSH
- DNA, Bacterial chemistry genetics MeSH
- Fluorescent Dyes chemistry MeSH
- Quantum Dots chemistry MeSH
- Humans MeSH
- Limit of Detection MeSH
- Oligonucleotide Probes chemistry genetics MeSH
- Salmonella typhi chemistry genetics isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Fluorescent Dyes MeSH
- Oligonucleotide Probes MeSH
The unavailability of simple, quick, and sensitive genetic-based molecular diagnostic techniques has become the main driving force for inventing new approaches in the era of quantum dots (QDs): a new class of fluorescent probes with fascinating optical electronic properties. Using the unique size-dependent light-emitting properties of QDs, we have developed a QD-based ultrasensitive technique which removes the necessity for the genetic amplification step required in almost all types of molecular-based diagnostic techniques. The selectivity of the new approach is warranted by the careful design of a pair of specific oligonucleotide probes, chemically modified at their 5'-ends. Our results indicated the selective detection of Salmonella typhi in an assay time of 50 min with a limit of detection (LOD) of 2 CFU/mL. The rapidity, selectivity, and sensitivity and the low assay cost make the new diagnostic technique a promising new tool for laboratory and field-based approaches to molecular diagnosis of health-threatening pathogens. Graphical abstract.
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