Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, technické zprávy
PubMed
29170991
DOI
10.1007/s12223-017-0567-y
PII: 10.1007/s12223-017-0567-y
Knihovny.cz E-zdroje
- MeSH
- agar chemie MeSH
- barvicí látky chemie metabolismus MeSH
- kultivační média chemie MeSH
- Paenibacillus růst a vývoj MeSH
- počet mikrobiálních kolonií metody MeSH
- polysacharidy metabolismus MeSH
- tetrazoliové soli chemie metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- technické zprávy MeSH
- Názvy látek
- agar MeSH
- barvicí látky MeSH
- kultivační média MeSH
- polysacharidy MeSH
- tetrazoliové soli MeSH
- triphenyltetrazolium MeSH Prohlížeč
Accurate enumeration of Paenibacillus mucilaginosus (formerly Bacillus mucilaginosus) bacterium from environmental samples on solid medium is challenging owing to its extensive extracellular polysaccharides (EPS) excretion. In the present study, P. mucilaginosus enumeration has been facilitated by a simple modification: addition of triphenyl tetrazolium chloride (TTC) to growth medium and application of a second soft agar layer. Results show distinctively better and accurate colonies' count. This method can be applied to all bacterial species that produce excessive EPS that may interfere with direct count.
Banat University of Agronomical Sciences and Veterinary Medicine Timișoara Romania
Shamir Research Institute University of Haifa P O Box 97 12900 Qatzrin Israel
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