Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium
Language English Country United States Media print-electronic
Document type Journal Article, Technical Report
PubMed
29170991
DOI
10.1007/s12223-017-0567-y
PII: 10.1007/s12223-017-0567-y
Knihovny.cz E-resources
- MeSH
- Agar chemistry MeSH
- Coloring Agents chemistry metabolism MeSH
- Culture Media chemistry MeSH
- Paenibacillus growth & development MeSH
- Colony Count, Microbial methods MeSH
- Polysaccharides metabolism MeSH
- Tetrazolium Salts chemistry metabolism MeSH
- Publication type
- Journal Article MeSH
- Technical Report MeSH
- Names of Substances
- Agar MeSH
- Coloring Agents MeSH
- Culture Media MeSH
- Polysaccharides MeSH
- Tetrazolium Salts MeSH
- triphenyltetrazolium MeSH Browser
Accurate enumeration of Paenibacillus mucilaginosus (formerly Bacillus mucilaginosus) bacterium from environmental samples on solid medium is challenging owing to its extensive extracellular polysaccharides (EPS) excretion. In the present study, P. mucilaginosus enumeration has been facilitated by a simple modification: addition of triphenyl tetrazolium chloride (TTC) to growth medium and application of a second soft agar layer. Results show distinctively better and accurate colonies' count. This method can be applied to all bacterial species that produce excessive EPS that may interfere with direct count.
Banat University of Agronomical Sciences and Veterinary Medicine Timișoara Romania
Shamir Research Institute University of Haifa P O Box 97 12900 Qatzrin Israel
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