Genetic characterization of phenicol-resistant Escherichia coli and role of wild-type repressor/regulator gene (acrR) on phenicol resistance
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
29307119
DOI
10.1007/s12223-017-0579-7
PII: 10.1007/s12223-017-0579-7
Knihovny.cz E-resources
- Keywords
- AcrAB efflux pump, Complementation, Phenicol, acrR mutation,
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Genes, Bacterial genetics MeSH
- DNA, Bacterial genetics MeSH
- Escherichia coli drug effects genetics isolation & purification MeSH
- Genotype MeSH
- Chickens MeSH
- Membrane Transport Proteins genetics MeSH
- Microbial Sensitivity Tests veterinary MeSH
- Drug Resistance, Multiple, Bacterial drug effects genetics MeSH
- Mutation MeSH
- Poultry Diseases microbiology MeSH
- Escherichia coli Proteins genetics MeSH
- Gene Expression Regulation, Bacterial MeSH
- Repressor Proteins genetics MeSH
- Chloramphenicol Resistance drug effects genetics MeSH
- Genetic Complementation Test veterinary MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- AcrR protein, E coli MeSH Browser
- Anti-Bacterial Agents MeSH
- DNA, Bacterial MeSH
- Membrane Transport Proteins MeSH
- Escherichia coli Proteins MeSH
- Repressor Proteins MeSH
The genetic basis for phenicol resistance was examined in 38 phenicol-resistant clinical Escherichia coli isolates from poultry. Out of 62 isolates, 38 showed resistance for chloramphenicol and nine for florfenicol, respectively. Each strain also demonstrated resistance to a variety of other antibiotics. Molecular detection revealed that the incidence rates of the cat1, cat2, flo, flo-R, cmlA, and cmlB were 32, 29, 18, 13, 0, and 0%, respectively. Nineteen strains were tolerant to organic solvents. PCR amplification of the complete acrR (regulator/repressor) gene of five isolates revealed the amino acid changes in four isolates. DNA sequencing showed the non-synonymous mutations which change the amino acid, silent mutation, and nucleotide deletion in four isolates. MY09C10 showed neither deletion nor mutation in nucleotide. The AcrA protein of the AcrAB multidrug efflux pump was overexpressed in these strains. Complementation with a plasmid-borne wild-type acrR gene reduced the expression level of AcrA protein in the mutants and partially restored antibiotic susceptibility one- to fourfold. This study shows that mutations in acrR are an additional genetic basis for phenicol resistance.
Department of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China
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