Impact of humic acid on the accumulation of metals by microalgae
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
VEGA 1/0164/17
Slovak Grant Agency
PubMed
29396826
DOI
10.1007/s11356-018-1362-2
PII: 10.1007/s11356-018-1362-2
Knihovny.cz E-zdroje
- Klíčová slova
- Bioaccumulation, Fluorescence microscopy, Heavy metals, Oxidative stress,
- MeSH
- chemické látky znečišťující vodu toxicita MeSH
- fluorescenční mikroskopie MeSH
- huminové látky analýza MeSH
- mikrořasy účinky léků MeSH
- oxidační stres MeSH
- reaktivní formy kyslíku metabolismus MeSH
- Scenedesmus účinky léků MeSH
- testy akutní toxicity MeSH
- těžké kovy toxicita MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chemické látky znečišťující vodu MeSH
- huminové látky MeSH
- reaktivní formy kyslíku MeSH
- těžké kovy MeSH
Indirect impact of humic acid (HA) on metal accumulation and toxicity (Cd, Ni, Pb, and Hg; 100 μM; 24 h of exposure) in Scenedesmus quadricauda was studied. Algae were pre-cultured on solid (10 and 100 mg HA/L) or in liquid media (1, 5, and 10 mg HA/L) over 30 days and then exposed to metals mentioned above. Accumulation of applied metals irrespective of pre-culture increased in the order Ni < Cd < Pb < Hg. Algae pre-cultured on solid HA-enriched media accumulated more Cd (+ 46% at 10 mg HA/L), Ni (+ 50 and + 81% at 10 and 100 mg HA/L, respectively), and Pb (+ 15% at 100 mg HA/L) but the impact on Hg amount was not detected. Potassium and calcium decreased in response to all metals (K strongly under Hg excess) and HA had negligible impact. Interestingly, fluorescence microscopy detection of reactive oxygen species/nitric oxide (ROS/NO) balance showed that HA pre-culture suppressed ROS signal and stimulated NO signal in response to Cd (indicating positive impact of HA) while ROS signal in Ni and Pb treatments rather increased but NO signal decreased as expected from elevated Ni and Pb accumulation. Hg had clearly the most toxic impact on the ROS/NO balance. Algae pre-cultured in liquid HA-enriched media showed significantly increased Ni accumulation only (+ 14% at a dose 10 mg HA/L). Present study for the first time showed that humic acid may indirectly affect accumulation of metals and that solid HA-enriched medium used for pre-culture is more suitable to increase accumulation of metals by algae.
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