Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
29432896
DOI
10.1016/j.tiv.2018.02.002
PII: S0887-2333(18)30038-9
Knihovny.cz E-resources
- Keywords
- Alternative fuels, DNA damage response, Gasoline exhaust particles, Gene expression profiling, Organic extracts,
- MeSH
- Gasoline analysis toxicity MeSH
- Biofuels analysis toxicity MeSH
- Cell Line MeSH
- Butanols analysis toxicity MeSH
- Ethanol chemistry MeSH
- Transcription, Genetic drug effects MeSH
- Air Pollutants analysis toxicity MeSH
- Humans MeSH
- MAP Kinase Signaling System drug effects MeSH
- Organic Chemicals chemistry toxicity MeSH
- Oxidative Stress drug effects MeSH
- Particulate Matter toxicity MeSH
- Lung drug effects pathology MeSH
- Polycyclic Aromatic Hydrocarbons analysis toxicity MeSH
- DNA Damage MeSH
- Gene Expression Profiling MeSH
- Vehicle Emissions analysis toxicity MeSH
- Inflammation chemically induced pathology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Gasoline MeSH
- Biofuels MeSH
- Butanols MeSH
- Ethanol MeSH
- Air Pollutants MeSH
- Organic Chemicals MeSH
- Particulate Matter MeSH
- Polycyclic Aromatic Hydrocarbons MeSH
- Vehicle Emissions MeSH
Modern vehicles equipped with Gasoline Direct Injection (GDI) engine have emerged as an important source of particulate emissions potentially harmful to human health. We collected and characterized gasoline exhaust particles (GEPs) produced by neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (n-But25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Despite the lowest GEP mass, n-But25 extract contained the highest concentration of polycyclic aromatic hydrocarbons (PAHs), while i-But25 extract the lowest. Gene expression analysis identified activation of the DNA damage response and other subsequent events (cell cycle arrest, modulation of extracellular matrix, cell adhesion, inhibition of cholesterol biosynthesis) following 4 h exposure to all GEP extracts. The i-But25 extract induced the most distinctive gene expression pattern particularly after 24 h exposure. Whereas E0, E15 and n-But25 extract treatments resulted in persistent stress signaling including DNA damage response, MAPK signaling, oxidative stress, metabolism of PAHs or pro-inflammatory response, i-But25 induced changes related to the metabolism of the cellular nutrients required for cell recovery. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content.
References provided by Crossref.org
Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface
