The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29554992
DOI
10.1017/s0031182018000409
PII: S0031182018000409
Knihovny.cz E-zdroje
- Klíčová slova
- Assemblage, Giardia intestinalis, clinical resistance, genotype, multilocus genotyping, subtypes,
- MeSH
- dítě MeSH
- dospělí MeSH
- genotyp MeSH
- Giardia klasifikace enzymologie genetika MeSH
- giardiáza parazitologie MeSH
- glutamátdehydrogenasa genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- multilokusová sekvenční typizace MeSH
- předškolní dítě MeSH
- protozoální proteiny genetika MeSH
- sekvenční analýza DNA MeSH
- triózafosfátizomeráza genetika MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- glutamátdehydrogenasa MeSH
- protozoální proteiny MeSH
- triózafosfátizomeráza MeSH
To date, genotyping data on giardiasis have not been available in the Czech Republic. In this study, we characterized 47 human isolates of Giardia intestinalis from symptomatic as well as asymptomatic giardiasis cases. Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (β-giardin, glutamate dehydrogenase and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and six (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterized by a large genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (including drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant associations with the clinical data were found.
Citace poskytuje Crossref.org
Clone-based haplotyping of Giardia intestinalis assemblage B human isolates