The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29554992
DOI
10.1017/s0031182018000409
PII: S0031182018000409
Knihovny.cz E-resources
- Keywords
- Assemblage, Giardia intestinalis, clinical resistance, genotype, multilocus genotyping, subtypes,
- MeSH
- Child MeSH
- Adult MeSH
- Genotype MeSH
- Giardia classification enzymology genetics MeSH
- Giardiasis parasitology MeSH
- Glutamate Dehydrogenase genetics MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Multilocus Sequence Typing MeSH
- Child, Preschool MeSH
- Protozoan Proteins genetics MeSH
- Sequence Analysis, DNA MeSH
- Triose-Phosphate Isomerase genetics MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glutamate Dehydrogenase MeSH
- Protozoan Proteins MeSH
- Triose-Phosphate Isomerase MeSH
To date, genotyping data on giardiasis have not been available in the Czech Republic. In this study, we characterized 47 human isolates of Giardia intestinalis from symptomatic as well as asymptomatic giardiasis cases. Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (β-giardin, glutamate dehydrogenase and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and six (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterized by a large genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (including drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant associations with the clinical data were found.
References provided by Crossref.org
Clone-based haplotyping of Giardia intestinalis assemblage B human isolates
