Transgenerational phenotype aggravation in CAF-1 mutants reveals parent-of-origin specific epigenetic inheritance
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Swiss National Science Foundation SNF - International
16-01137S
Czech Science Foundation - International
Swedish Science Foundation - International
Knut-and-Alice-Wallenberg Foundation - International
PubMed
29573427
DOI
10.1111/nph.15082
Knihovny.cz E-resources
- Keywords
- Arabidopsis thaliana, CAF-1, Chromatin, DNA methylation, Development, epigenetics, histone,
- MeSH
- Alleles MeSH
- Arabidopsis genetics MeSH
- Epigenesis, Genetic * MeSH
- Phenotype MeSH
- Stress, Physiological genetics MeSH
- Gene Ontology MeSH
- DNA Methylation genetics MeSH
- Mutation genetics MeSH
- Plant Infertility MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Base Sequence MeSH
- Seeds embryology MeSH
- RNA Splicing Factors genetics metabolism MeSH
- Transcriptome genetics MeSH
- Inheritance Patterns genetics MeSH
- Ovule embryology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- At2g20020 protein, Arabidopsis MeSH Browser
- FAS protein, Arabidopsis MeSH Browser
- Arabidopsis Proteins MeSH
- RNA Splicing Factors MeSH
Chromatin is assembled by histone chaperones such as chromatin assembly factor CAF-1. We had noticed that vigor of Arabidopsis thaliana CAF-1 mutants decreased over several generations. Because changes in mutant phenotype severity over generations are unusual, we asked how repeated selfing of Arabidopsis CAF-1 mutants affects phenotype severity. CAF-1 mutant plants of various generations were grown, and developmental phenotypes, transcriptomes and DNA cytosine-methylation profiles were compared quantitatively. Shoot- and root-related growth phenotypes were progressively more affected in successive generations of CAF-1 mutants. Early and late generations of the fasciata (fas)2-4 CAF-1 mutant displayed only limited changes in gene expression, of which increasing upregulation of plant defense-related genes reflects the transgenerational phenotype aggravation. Likewise, global DNA methylation in the sequence context CHG but not CG or CHH (where H = A, T or C) changed over generations in fas2-4. Crossing early and late generation fas2-4 plants established that the maternal contribution to the phenotype severity exceeds the paternal contribution. Together, epigenetic rather than genetic mechanisms underlie the progressive developmental phenotype aggravation in the Arabidopsis CAF-1 mutants and preferred maternal transmission reveals a more efficient reprogramming of epigenetic information in the male than the female germline.
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