Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro : Effect of Some Growth Factors and Cytokines
Language English Country United States Media print-electronic
Document type Journal Article
Grant support
0115U000355
National Academy of Sciences of Ukraine (UA)
LM2015055
Czech Research Infrastructure for Systems Biology C4SYS
PubMed
29589213
DOI
10.1007/s10528-018-9854-9
PII: 10.1007/s10528-018-9854-9
Knihovny.cz E-resources
- Keywords
- Antibody, Cytokine, DNA damage, Growth factor, Human cells in vitro, Repair enzyme MGMT,
- MeSH
- Cell Culture Techniques MeSH
- Cell Line MeSH
- Hep G2 Cells MeSH
- Cytokines pharmacology MeSH
- DNA Modification Methylases genetics MeSH
- DNA Repair Enzymes genetics MeSH
- Humans MeSH
- Intercellular Signaling Peptides and Proteins pharmacology MeSH
- Tumor Suppressor Proteins genetics MeSH
- Gene Expression Regulation drug effects MeSH
- In Vitro Techniques MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cytokines MeSH
- DNA Modification Methylases MeSH
- DNA Repair Enzymes MeSH
- Intercellular Signaling Peptides and Proteins MeSH
- MGMT protein, human MeSH Browser
- Tumor Suppressor Proteins MeSH
The inducible repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates O6-methylguanine adducts in DNA and protects the cells from damaging effects of alkylating agents. We have found that anti-MGMT antibodies recognize both the MGMT protein with a mol. weight ~ 24 kDa and a protein with a mol. weight ~ 48 kDa, which was named MARP (anti-methyltransferase antibody recognizable protein). A number of growth factors and cytokines were shown to regulate the expression of MGMT and MARP proteins. The ranges of concentrations of several growth factors and cytokines that caused increasing or decreasing protein amounts in human cell cultures were determined. The results of special biological experiments have allowed us to assume a possible role of MARP in the repair of alkyl adducts in human cells.
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