Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro : Effect of Some Growth Factors and Cytokines
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
0115U000355
National Academy of Sciences of Ukraine (UA)
LM2015055
Czech Research Infrastructure for Systems Biology C4SYS
PubMed
29589213
DOI
10.1007/s10528-018-9854-9
PII: 10.1007/s10528-018-9854-9
Knihovny.cz E-zdroje
- Klíčová slova
- Antibody, Cytokine, DNA damage, Growth factor, Human cells in vitro, Repair enzyme MGMT,
- MeSH
- buněčné kultury MeSH
- buněčné linie MeSH
- buňky Hep G2 MeSH
- cytokiny farmakologie MeSH
- DNA modifikační methylasy genetika MeSH
- enzymy opravy DNA genetika MeSH
- lidé MeSH
- mezibuněčné signální peptidy a proteiny farmakologie MeSH
- nádorové supresorové proteiny genetika MeSH
- regulace genové exprese účinky léků MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cytokiny MeSH
- DNA modifikační methylasy MeSH
- enzymy opravy DNA MeSH
- mezibuněčné signální peptidy a proteiny MeSH
- MGMT protein, human MeSH Prohlížeč
- nádorové supresorové proteiny MeSH
The inducible repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates O6-methylguanine adducts in DNA and protects the cells from damaging effects of alkylating agents. We have found that anti-MGMT antibodies recognize both the MGMT protein with a mol. weight ~ 24 kDa and a protein with a mol. weight ~ 48 kDa, which was named MARP (anti-methyltransferase antibody recognizable protein). A number of growth factors and cytokines were shown to regulate the expression of MGMT and MARP proteins. The ranges of concentrations of several growth factors and cytokines that caused increasing or decreasing protein amounts in human cell cultures were determined. The results of special biological experiments have allowed us to assume a possible role of MARP in the repair of alkyl adducts in human cells.
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