GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Chloroformate derivatization, Dispersive liquid-liquid microextraction, GC-MS, Metabolomic profiling, Quantitative analysis, Urine,
- MeSH
- Urinalysis methods MeSH
- Biomarkers urine MeSH
- Fluorocarbons chemistry MeSH
- Formates chemistry MeSH
- Humans MeSH
- Metabolomics methods MeSH
- Liquid Phase Microextraction methods MeSH
- Gas Chromatography-Mass Spectrometry methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Fluorocarbons MeSH
- Formates MeSH
- heptafluorobutyl chloroformate MeSH Browser
A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
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