Molecular Evidence of Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi in Red Foxes ( Vulpes vulpes)
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29733765
DOI
10.7589/2017-09-240
Knihovny.cz E-zdroje
- Klíčová slova
- Carnivores, PCR, encephalitozoonosis, neosporosis, toxoplasmosis,
- MeSH
- divoká zvířata MeSH
- Encephalitozoon cuniculi genetika izolace a purifikace MeSH
- encephalitozoonóza epidemiologie mikrobiologie veterinární MeSH
- genotyp MeSH
- kokcidióza epidemiologie parazitologie veterinární MeSH
- lišky parazitologie MeSH
- Neospora genetika izolace a purifikace MeSH
- Toxoplasma genetika izolace a purifikace MeSH
- toxoplazmóza zvířat epidemiologie parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi are important infectious agents, with T. gondii and E. cuniculi having zoonotic potential. There are two main clonal lineages (types I and II) of T. gondii in Europe, but little is known about genotypes of T. gondii in wild animals. The aim of our study was molecular detection of these three pathogens in tissues of wild red foxes ( Vulpes vulpes) from the Czech Republic. Using PCR (B1 gene), we detected T. gondii in 10% of the animals that we tested ( n=100); N. caninum and E. cuniculi were not detected. The T. gondii samples were genotyped by single multiplex PCR assay with 15 microsatellite markers. Five samples were successfully genotyped as genotype II, a unique finding for T. gondii isolated from red foxes from the Czech Republic.
INSERM UMR S 1094 Neuroépidémiologie Tropicale Université de Limoges 87025 Limoges France
State Veterinary Institute Prague Sídlištní 136 24 165 03 Prague 6 Czech Republic
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